Paula in PA Posted November 28, 2012 Share Posted November 28, 2012 I have a full bottle of agar but only need part of it at this time. Can I heat it up, use what I need, then let the rest of the bottle cool and re-solidify to be used later? TIA Quote Link to comment Share on other sites More sharing options...
chirowife Posted November 29, 2012 Share Posted November 29, 2012 most likely once you open it it will no longer be sterile and will grow stuff... Quote Link to comment Share on other sites More sharing options...
RootAnn Posted November 29, 2012 Share Posted November 29, 2012 I have agar in my fridge that is fine (not growing stuff) that we used some of last year. Just keep it in the fridge & be careful how you pour it. Don't touch the lip/lid to anything. Use gloves, etc. Good luck! Quote Link to comment Share on other sites More sharing options...
NittanyJen Posted November 30, 2012 Share Posted November 30, 2012 Yes, you can reuse agar, reheat it, let it solidify, and reuse it. Just be careful to use sterile technique when pouring. It will help if when you pour, you will naturally have the vessel at an angle-- only tip it up enough to stop the liquid from pouring, but do not tip it upright any farther; cap it off as soon as it stops pouring. Preferably, hold the cap between your fingers rather than setting it down on the counter while pouring. If you must set it down, set it down on a clean lab diaper or paper towel, facing up. (The face up may be counter-intuitive; you may be worried about germs "falling into the cap," but it is more important that you not set it down face down and pick up germs around the rim by contact with the counter surface). Even better, use gloves when handling the container and pouring. Quote Link to comment Share on other sites More sharing options...
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