HSMWB Posted March 19, 2015 Share Posted March 19, 2015 I purchased a bacteria culturing kit evidently about a year ago, one from home science tools. Have not used it (kept waiting till the 'right time'), it has sat in a cardboard box in a cupboard in the family room - climate controlled - this whole time. The bottle nutrient agar bottle says it expires 3/14/14. It 'looks' ok to me. I finally want to use it for a science 'camp' in a week and a half. Do you think it would still work? Would you use it? I'm afraid that if I order more it probably will not come in time. Use it or skip this experiment???? WWYD Quote Link to comment Share on other sites More sharing options...
Jean in Newcastle Posted March 19, 2015 Share Posted March 19, 2015 If it is not discolored and still is free flowing, then I'd use it. But I would recognize that the experiment might not work. 1 Quote Link to comment Share on other sites More sharing options...
duckens Posted March 19, 2015 Share Posted March 19, 2015 I'd use it for my family, but not depend on it for a camp. Loverboy made agar with his niece using knox box for her science fair. A year later, they banned "live animals" from the science fair....probably because they were worried someone would show up with a major biological hazard.Disclaimer: I don't know if any of this will work:Loverboy says to use chicken broth in Knox blox. Here is one website that talks about it.ETA: Loverboy also says the tough part is keeping the plates the right temperature (warm enough). Quote Link to comment Share on other sites More sharing options...
Syllieann Posted March 20, 2015 Share Posted March 20, 2015 If it looks ok and is still sealed I would use it. It sounds like all your cultures will be using the same agar. I wouldn't try to compare growth in that agar vs some other bacterium on a different agar. Companies need to put expiration dates on things for a variety of reasons and they are usually very conservative. In some cases, it is simply a date after which they don't have to track products for recall. The worst case scenario here is not very bad; maybe you will experience reduced growth or more difficulty in plating. If you can live with that, go for it. 1 Quote Link to comment Share on other sites More sharing options...
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