Would you be willing to give the master bedroom to the youngest 4? Then the 3 oldest boys in room 3, 2 older girls in room 2, you in room 1. It often makes more sense for the adults to take a smaller room since there is less furniture / stuff / need for more space and it's big enough for more kids to share.
If that wouldn't work, I'd probably switch the 3 oldest boys and 3 youngest girls, give the oldest girl the den back and try to find somewhere else for the sewing machine either your bedroom or the den.